Anti-MCL1 Rabbit Monoclonal Antibody

Product Name

Anti-MCL1 Rabbit Monoclonal Antibody

View all Mcl-1 Antibodies

SKU/Catalog Number

M00712

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-MCL1 Rabbit Monoclonal Antibody catalog # M00712. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MCL1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00712)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BCO-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human MCL1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00712 is reactive to MCL1 in Human, Mouse, Rat

Applications

M00712 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

37.337kDa

Background of Mcl-1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MCL1 using anti-MCL1 antibody (M00712).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCL1 antigen affinity purified monoclonal antibody (Catalog # M00712) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCL1 at approximately 37 kDa. The expected band size for MCL1 is at 37 kDa.

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Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded human stomach, using MCL1 Antibody.

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

Gene/Protein Information For MCL1 (Source: Uniprot.org, NCBI)

Alternative Names

BCL2L3; bcl2-L-3; BCL2L3MGC104264; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; EAT; induced myeloid leukemia cell differentiation protein Mcl-1; Mcl1; Mcl-1; mcl1/EAT; MCL1-ES; MCL1L; MCL1S; MGC1839; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related); TM MCL1 BCL2L3, EAT-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT, MCL1 MCL1 apoptosis regulator, BCL2 family member induced myeloid leukemia cell differentiation protein Mcl-1|BCL2 family apoptosis regulator|MCL1, BCL2 family apoptosis regulator|bcl-2-like protein 3|bcl-2-related protein EAT/mcl1|myeloid cell leukemia 1|myeloid cell leukemia ES|myeloid cell leukemia sequence 1 (BCL2-related)

For more info on MCL1, check out the MCL1 Infographic

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In this infographic, you will see the following information for MCL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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